2-tetrahydrofurfuryl-, 2-aliphatic-, and 2-aliphatic ether-sulfonamido-5-alkoxy-pyrimidines



United States Patent C) M Ser. No. 514,156

17 Claims. (Cl. 260-2565) This application is a division of copending application, Serial Number 144,530, filed October 12, 1961.

This invention relates to compounds capable of lowering the blood sugar level in warm blooded animals, and more particularly to sulfonamide compounds capable of such a physiological effect.

sulfonamide compounds which lower the blood sugar level and are suitable for the treatment of diabetes mellitus have been known for about two decades. Some compounds of the sulfonamide type have found general acceptance for this purpose. We now have found a new group of sulfonamide compounds which compare favorably in their blood sugar level depressing effect and their low toxicity with the best known physiologically effective sulfonamide agents. The sulfonamide compounds of this invention are effective when administered orally, their effects are relatively long-lasting, and their toxicity is low. The toxicity of some of our novel compounds is lower than that of the best heretofore available blood sugar level depressants which makes them particularly valuable for long term application.

The novel compounds of the invention have the general formula N=CH (1) wherein A is a phenyl radical which may carry one or two side chains but should not be linked to a nitrogen atom in the para-position; A may also be a saturated or unsaturated hydrocarbon radical having a straight, branched, or cyclic carbon chain, or a carbon chain interrupted by one or more interposed oxygen atoms. B is the group X-R, wherein X is either a bond between adjacent carbon atoms or an oxygen atom, and R is a saturated or unsaturated hydrocarbon radical which may have a straight, branched, or cyclic carbon chain, or a carbon chain interrupted by one or more interposed oxygen atoms.

The compounds of the invention may be prepared by several methods such as the following:

METHOD I Compounds of the formulas A--SO Hal and (A40 O (2) wherein Hal is a halogen, and m is an integer between and 2, are reacted with a 2-amino-5-B-pyrimidine. A and B represent the same radicals as in Formula 1. The reaction product may have to be oxidized to the sulfonic acid derivative when m is smaller than 2.

METHOD II A sulfonamide compound of the formula ASO NH preferably in the form of its alkali metal salt, is reacted with a Z-halogeno-S-B-pyrimidine.

3,288,793 Patented Nov. 29, 1966 is condensed to form a cyclic structure with a compound of the formula 'z*- 1H' z' wherein Z and Z are each either hydrogen or an alkoxy radical. If Z or Z is hydrogen, and the compound therefore is an aldehyde, a corresponding reactive aldehyde derivative may be employed as the starting material. If the reaction product carries hydroxyl groups in positions 4 and/or 6 of the pyrimidine ring, these groups are replaced by hydrogen in the usual manner.

METHOD V A sulfonamide of the formula is reacted with a product obtained by the Vilsmeier reaction from a 1,l-dialkoxy-Z-B-Z-ethane or a 1-B-1 alkoxy ethylene with an N,N-d-isubstituted formamide in the presence of the chloride of an inorganic acid such as phosphorus pentachloride or phosgene. If B in the 1,1-dialkoxy-Z-B-ethane or the l-B-Z alkoxy ethylene is an alkoxy radical, the other alkoxy radicals may be the same as B. The Vilsmeier reaction product may, for example, be ,li-dialkylamino-a-B-acnoleine and may be used as its acetal. The aforementioned sulfonamide may also be reacted with a fialkoxy-a-B-acroleine or its acetal, or with ii-chloro-a-B-acroleine or its acetal.

METHOD VI A sulfonamide of the formula ASO NH (3) is reacted with a Z-nitroaminoor 2-cyanamino-5-B- pyrimidine.

METHOD VII A sulfonic acid of the formula Afiso -on ('6) is reacted according to Freudenbe-rgs method with a 2-acetylamino-S-B-pyrimidine.

METHOD VIII A sulfonyl halide of the formula 3 METHOD IX The aminopyrimidinederivatives employed, for example, in Method I are preferably prepared by reacting a 1,1-dialkoxy-2-B-ethane derivative of the formula BGH2CH D wherein D and D" are lower alkyl radicals with an acidic halogen bearing condensation agent suitable for the V118- meier reaction, such as phosphorous pentachloride, so as to replace one of the alkoxy radicals in Formula 8, and reacting the compound formed with a formamide of a secondary amine of the formula \NCHO D which is added either simultaneously with the Vilsmeier condensation agent or thereafter, and wherein D is an alkyl radical, and D is an alkyl or phenyl radical. A preferred example of an amine of Formula 9 is dimethylformamide. The thus obtained Vilsmeier condensation reaction product is then reacted with a solution of a guanidine salt in the presence of an alkali metal hydroxide or of an alkali metal ialcoholate dissolved in a lower alkanol. The following examples are further illustrative of the present invention, and it will be understood that the invention is not limited thereto:

Example 1 210 g. phosphorus pentachloride are gradually added to 252 g. methoxyethoxyacetaldehyde-di-methoxyethylacetal with agitation. The mixture is externally cooled with ice to hold the reaction temperature below 25 C. Moisture is carefully excluded. After addition of the condensation agent is completed, the reaction mixture is further agitated at room temperature for 30 minutes. 225 ml. dimethylformamide are then added drop by drop while the reaction temperature is held at 20 to 25 C. by external cooling of the reaction vessel with ice. When the dimethylformamide has been added, the temperature 15 raised to 60 C., and this temperature is maintained for 70 minutes.

I The temperature is again lowered to 20-25" C. and maintained at this value by cooling with ice While 500 ml. methanol are added drop by drop. The resulting solution is admixed drop by drop to a suspension of 240 g. powdered caustic soda in 800 ml. methanol at 20 to 25 C. After mixing is completed, stirring is continued for 30 minutes at room temperature. The solution now contains inorganic salts and [3-dimethylamino-u-methoxyethoxyacroleine.

200 g. guanidine nitrate and thereafter 70 g. sodium hydroxide are added to the'solution. The methanol is evaporated with agitation. The residue is dissolved in 1.5 liter water and is repeatedly extracted with chloroform. The combined chloroform extracts are evaporated to dryness, and the residue is recrystallized from carbon tetrachloride. 80 grams of 2-amino-S-methoxyethoxypyrimidine of M.P. 808l C. are obtained.

Example 2 324 g. 1,1,2-tricyclohexyloxyethane when subjected to the sequence of steps of Example 1 instead of methoxyethoxyaeetaldehyde-di-methoxyethylacetal yield 88 g. 2- amino-5-cyclohexyloxypyrimidine of M.P. 72-73" C.

Example 3 430 g. ethoxyethoxyethoxyacetaldehyde di ethoxyethoxyethylacetal are reacted with phosphorus pentachloride, dimethylformamide, and guanidine nitrate in the same manner as set forth in Example 1. The crude base obtained by the chloroform extraction is dissolved in dilute hydrochloric acid and the acid solution is repeatedly ex-.

tracted with ether. The aqueous phase is made alkaline with sodium hydroxide and is extracted with chloroform. The chloroform extract is evaporated and 175 g. Z-amino- 5-ethoxyethoxyethoxypyrimidine are obtained as a brown oil.

The base is transformed for identification into the corresponding benzenesulfonyl compound of M.P. 118-119 C. by reaction with benzenesulfochloride in pyridine solution.

Example 4 330 g. tetrahydroturfuryloxyacetaldehyde-di-tetrahydrofurylacetal when subjected to the reaction sequence of Example 3 yield 130 g. 2-amino-5-tetrahydrofurfuryloxypyrimidine which is a brown oil. The corresponding benzenesulfonyl derivative melts at 213-215 C.

In an analogous manner there are prepared 2-amino-5- B-pyrimidines wherein B represents the following radicals:

Methoxyethoxy, methyl, n-propoxy-, ethyl, ethoxy, methoxy-methyl, ethoxymethyl, isopropoxy, and isobutoxy.

The guanidine nitrate mentioned in Examples 1 to 4 may be replaced by another guanidine salt which reacts as the free guanidi-ne base in an alkaline medium.

Example 5 15 g. calcium carbonate and 20 g. toluenesulfochloride are added to a solution of 12.5 g. 2-amino-5-methoxypyrimidine in 100 ml. Water. The mixture is gradually heated to 80 C. within 30 minutes, and held at that temperature for two hours while it is being agitated. At the end of this period, the toluenesulfochloride is no longer visible as a separate phase. 20 ml. 30% sodium hydroxide solution are added, and the solution is clarified by tained is poured into 300 ml. water.

the addition of charcoal and filtering. Upon addition of concentrated hydrochloric acid 21.5 g. 2-(4-methylbenzenesulfonamido)-5-methoxypyrimidine of M.P. 196- 197 C. are obtained.

Example 6 15.3 g. Z-amino-5-n-propoxypyrimidine are dissolved in 60 ml. pyridine and are agitated jointly with 19 g. toluenesulfochloride for two hours at 60 C. The solution ob- The precipitate formed is filtered and washed with water. The insoluble material is dissolved in 300 ml. N sodium hydroxide, and charcoal is added to the resulting solution which is then filtered. A precipitate formed in the filtrate by the addition of hydrochloric acid is 2 (4-methylbenzenesulfonamido)-5-n-propoxy-pyrimidine of M.P. 198200 C. The yield is 29 grams.

Example 7 A mixture of 18 g. of the sodium salt of 4-methoxybenzenesulfonamide and 14.5 g. 2-chloro-5-methoxypyrimidine is finely ground and heated with agitation for four hours to C. A homogeneous melt is formed. It solidifies on cooling and is dissolved in 300 ml. N sodium hydroxide solution. The solution is purified by the addition of charcoal and filtering. 23.5 g. 2-(4- methoxybenzenesulfonamido) 5 --methoxypyrimidine of M.P. 153154 C. are precipitated from the filtrate by the addition of concentratedhydrochloric acid.

Example 8 2-(4-methylbenzenesulfonamido)-5-methoxypyrimidine of M.P. 196-197" C. are obtained.

Example 10 12 g. 1,1,2trimethoxyethane are mixed with 21 g. phosphorus pentachloride. The reaction mixture is kept at a temperature of 22 to 25 C. by cooling. The mixture is stirred for 30 minutes at room temperature, whereafter 22.5 ml. dimethylformamide are added drop by drop while the mixture is being cooled to 2025" C. After the addition of dimethylformamide is completed, the mixture is heated to 40 C. whereby an exothermic reaction is started. The temperature is held for 75 minutes at 60 C. The reaction mass is then permitted to cool, and 50 m1. methanol are added drop by drop while room temperature is maintained.

The solution formed is gradually admixed at 20 to 25 C. to a suspension of 24 g. powdered sodium hydroxide in 80 ml. methanol, and the mixture obtained is stirred for 30 minutes. It noW contains inorganic salts and p-dimethylamino-a-methoxyacroleine. The latter is further reacted by the addition of 20 g. toluenesulfoguanidine and refluxing of the solution for eight hours with agitation. The reaction mixture is worked up in a manner analogous to the procedure of Example 9. The compound obtained is recrystallized from acetone. 18.2 g. 2 (4-methylbenzenesulfonamido)-5-methoxy-pyrimidine of M.P. 196197 C. are obtained.

Example 11 25 g. 2-amino-5-methoxypyrimidine are dissolved in 250 ml. anhydrous benzene. A solution of 16 g. ptoluenesulfenylchloride in 150 ml. benzene is added drop by drop with cooling. The completed mixture is stirred 30 minutes at 30 C. Upon cooling to room temperature crystals are precipitated. They are filtered off with suction and permitted to dry in air. The dry crystals are stirred with 100 ml. Water and filtered by means of suction to remove coprecipitated 2-amino-5-methoxy-pyrimidine hydrochloride. The moist solid on the filter is dissolved in 300 ml. glacial acetic acid and is oxidized in the solution by the addition of 60 ml. 30% hydrogen peroxide. Oxidation is complete after 12 hours, and the solution is evaporated in a vacuum to a volume of 100 ml. Upon dilution with 300 ml. water a crystalline precipitate is formed which is filtered off and recrystallized from 50% acetone.

21.5 g. 2-(4-methylbenzenesulfonamido)-5 methoxypyrimidine of M.P. 196-197 C. are obtained.

Example 12 25 g. 2-amino-5-methoxypyrimidine are dissolved in 300 ml. benzene, and the solution is added at C. to 22 g. toluenesulfinyl chloride dissolved in 100 ml. benz'ene. The mixture is stirred two hours at room temperature. A mixture of 2-(4-methylbenzenesulfinamido)-5- methoxypydimidine and 2-amino-methoxypyrimidine hydrochloride is precipitated. The precipitate is filtered ofi, digested with Water and oxidized in the same manner as described in Example 11. The yield is 23 g. 2- (methylbenzenesulfonamido) S-methoxy-pyrimidine of M.P. 196197 F.

Example 13 17.7 g. 2-amino-5-cyclohexyloxypyrimidine are dissolved in 60 ml. pyridine. 17 g. benzenesulfochlor-ide 6 are added and the resulting mixture is heated two hours to 60 C. It is then poured into 300 ml. water. The precipitate formed thereby is filtered off and dissolved in dilute ammonium hydroxide. The solution is purified with charcoal, and filtered. The filtrate is acidified with acetic acid. 30 g. 2-benzenesulfonamido-S-cyclohexyloxypyrimidine of M.P. 225-226" C. are obtained.

Example 14 12.5 g. 2-amino-S-methoxypyrimidine are dissolved in 60 ml. pyridine and mixed with 17 g. isoamylsulfochloride. The mixture is stirred for three hours at room temperature, and then poured into 300 ml. water. The precipitate formed is filtered off, washed with water, and dissolved in dilute ammonium hydroxide solution. The solution so obtained is clarified by addition of charcoal and filtration. 20 g. 2-isoamylsulfonamido-5-rnethoxypyrimidine are precipitated from the filtrate by the addition of acetic acid. M.P. 163-165 C.

Example 15 12.5 g. 2-amino-5-methoxypyrimidine are dissolved in pyridine and the solution is stirred with 18 g. ethoxyethylsulfochloride for three hours at room temperature. The pyridine is distilled off in vacuo and the residue is stirred with 200 ml. water for one hour. The insoluble material is filtered oil and purified by reprecipitation from dilute ammonium hydroxide solution with acid. 20 g. 2-ethoxyethylsulfonarnido-S-methoxypyrimidine of M.P. 124-l25 C. are obtained.

Example 16 21 g. cyclohexylsulfoguanidine of M.P. 291 C. are prepared in a conventional manner from guanidine and cyclohexylsulfochloride. The cyclohexylsulfoguanidine and 6 g. sodium methylate are added to a solution of 13 g. B-dimethylamino-a-methoxyacrolein in 250 ml. methanol. The mixture is distilled off. The residue is dissolved in water, and the aqueous solution is acidified with acetic acid. The precipitate formed thereby is filtered off by means of suction, washed with water, and dissolved in dilute ammonium hydroxide solution. The ammoniacal solution is purified by addition of charcoal and filtration, and acidified with acetic acid. 2-cyclohexylsulfonamido 5 methoxypyrimidine is precipitated. The yield is 22 g., the melting point 23l-235 C.

Example 17 14 g. isoamylsulfenyl chloride dissolved in ml. anhydrous ether are added drop by drop to a solution of 25 g. 2-amino-5-methoxypyrimidine in 300 ml. anhydrous ether. The reaction mixture is stirred for 30 minutes while it is being kept at its boiling point. A mixture of 2-isoamylsulfeneamido-S-methoxypyrimidine and 2-aminO-S-methoxypyrimidine hydrochloride is precipitated. The precipitate is digested with 100 ml. water and filtered. The solid fraction is dissolved in 200 ml. glacial acetic acid and oxidized by the addition of 60 ml. 30% hydrogen peroxide. After 24 hours standing at room temperature, the solution is evaporated to a volume of about 50 ml. and then diluted with 200 ml. water. A crystalline precipitate of 2-is0amylsulfonamido-5-methoxypyrimidine is formed which is filtered olt and re crystallized from 50% ethanol. The yield is 12 g., the melting point 163-165 C.

Example 18 25 g. 2-amino-5-ethoxypyrirnidine are dissolved in 200 ml. anhydrous ether and are mixed at 5 C. with a solution of 15 g. isoamylsulfinyl chloride in 100 ml. anhydrous ether. The mixture is stirred for two hours at room temperature. The precipitate formed is filtered off and oxidized with hydrogen peroxide as described in Example 13. The sulfonamide derivative obtained is recrystallized from 50% acetone. There are obtained 14 g. iso amylsulfonamido-S-ethoxypyrirnidine of MP. 17-0-172".

Example 19 20 g. isoamylsulfogruanidine of M.P. 198 C. are prepared by re-acting guanidine with isoamylsulfochloride. The isoamylsulfoguanidine is heated with 12 g. sodium methylate and 16 g. of the dimethyl ester of methoxymalonic acid in 200 ml. methanol for four hours in an autoclave to 110 C. Upon cooling of the reaction mixture, the methanol is evaporated and the residue is dissolved in 200 ml. Water. When the aqueous solution is acidified with hydrochlonic acid, 25 g. 2-isoamy1sulfionamid- 4, 6-dihydroxy-S-methoxypyrimidine are obtained. The precipitated compound is refluxed without further purification with 150 ml. phosphorus oxychloride for 30 minutes. The excess of phosphorus oxychloride is distilled off in a vacuum. The residue is poured on ice and stirred therewith for one hour. The aqueous solution obtained is purified by treatment with charcoal and filtnation. The filtrate is adjusted to a pH value of 4-5 by the addition of sodium acetate, and further stirred for about two hours. 2-isoamylsulfonamido-4,5-dichloro-5- methoxypyrimidine is precipitated, filtered oif, and dissolved in 300 mi. N sodium hydroxide solution. The

at C. Upon completion of the reduction, the mixture is filtered while still hot to remove the insoluble zinc salts. The filtrate is acidified with acetic acid and 18 g. Z-isoamylsulfonarnido-S-rnethoxypyrimidine are obtained. The melting point is 163-165 C. after recrystallization from 50% ethanol.

The reduction of the dichloropyrimidine derivative and the fourth column lists the melting point in degrees solution is agitated with 20 g. zinc dust for two hours Centigrade.

' TABLE 1 Method of A X-R M1 Ex. C.

a mo- 0C2H5 201-203 CH: 5 H1O -0on 203-209 6 moQ- -o-n-otm -183 6 moQ- -OH.-, 256-260 6 H5O o-- -o1u 235-240 6 HaQQ- 'C2H5 218-219 6 H3O 0 -01H5 191-195 6 o1- -OCH3 ass-134 6 C1 -OCJH5 184-185 a o1- -On-CaH1 188-190 OH: e 01 -oon 196-200 6 OCH: 172-174 6 On-OaH1 184-185 CH: 6 H300 -0 011 185-186 TABLE IContinuec1 Method of A Example 20 Example 21 61 g. 2-benzenesulfonamido 5 methoxyethoxypyrimidine are dissolved in a hot solution of 7.4 g. calcium hydroxide in 250 ml. water. The solution is filtered and evaporated to dryness in a vacuum. The residue is digested with 300 ml. acetone and filtered. The insoluble fraction is dried and consists of 60 g. of the calcium salt of Z-benzenesulfonamido 5 methoxyethoxy-pyrimidine, melting at about 150 C.

Example 22 25 g. Z-benzenesulfonamido-S-methylpyrimidine are dissolved jointly with 15 g. ethanolamine in 300 ml. hot ethanol. A precipitate forms when the solution is permitted to cool. It is filtered off and washed with ethanol. After drying at about 50 C., 26 g. of the ethanolamine salt of 2-benzenesulfonamido-S-methylpyrimidine are obtained, having a melting point of 138l40 C.

Example 23 5 kg. 2-benzenesulfonamido-5-rnethylpyrimidine are worked into a paste according to conventional pharmaceutical procedures by mixing with an aqueous dispersion of polyvinylpyrrolidone and corn starch containing 0.1% of methyl p-methoxybenzoate or 0.1% of propyl p-methoxybenzoate as a preservative. The paste is dried and granulated. 80 g. talcum powder and additional corn starch are added to make the total weight 6.2 kg. The material is ready to be tabletted on conventional presses. Individual tablets each having a weight of 620 mg. and containing 500 mg. of the active sulfonamide material are pressed.

Example 24 The calcium salts of the sulfonamide compounds of our invention are readily soluble in water. For oral application of the sulfonarnide compounds of our invention, we fill standard gelatine capsules (size with the calcium salt of Z-benzene sulfonamido-S-methoxypyrimidine.

Example 25 10 kg. Z-benzenesulfonamido-S-n-propoxypyrimidine are micronized to a particle size of to microns. The fine powder is suspended by mechanical agitation in a carrier liquid of the following composition:

Distilled water liters 73 Hydroxyethylcellulose kg 1.1 Sorbic acid g 150 Sodium lauryl sulfate g 50 Fruit extract (flavoring) kg 1 The total volume is adjusted with distilled water to liters kg.). The liquid obtained contains in one teaspoonful (about 5 ml.) approximately 500 mg. of the active sulfonamide compound.

Example 26 If it is desired to apply the sulfonamide compounds of the invention externally, a solution may be prepared according to the following method:

21.46 g. 2 benzenesulfonamido 5 methoxy-ethoxypyridimidine sodium corresponding to 20 g. of. the free sulfonamide compound are dissolved in 100 ml. distilled water. 1 ml. of the resulting solution contains 200 mg. of the active ingredient. The solution may be sterilized in the usual manner if this should be necessary.

It will be understood that Examples 20 and 21 are merely representative of methods for preparing salts of the sulfonamide compounds of the invention, and that the specific pyrimidine derivatives mentioned in the examples may be replaced by any one of the other pyrimidine derivatives disclosed. The potassium, lithium, ammonium salts are closely similar to the sodium salts and may be prepared by analogous methods. Equally, other physiologically tolerated amines may be substituted for the ethanolamine salt specifically mentioned in Example 22, and methylglucamine, morpholine, piperazine, are additional examples of such organic bases. Where desired or necessary, the solubility of the sulfonamide compounds of the invention may also be increased 'by the admixture or joint application of bicarbonates or carbonates of the alkali metals or of ammonium in a manner well understood by those skilled in the art.

The potassium, strontium, and barium salts are readily prepared by the method of Example 20, but they are not of pharmacological interest. They may serve as intermediates in the production of salts with other bases, particularly organic bases.

The desirable pharmacological properties of the novel sulfonamide compounds of this invention will become more fully apparent from the Table II in which the median lethal dosis LD is listed for representative sulfonamide compounds of this invention together with the percentage reduction in blood sugar level produced.

The median lethal dosis LD was determined by intravenous injection in rats. The percentage reduction in blood sugar level was tested in fasting rabbits after oral ingestion of 500 mg./kg. of the active ingredient. For comparison purposes, the same tests were performed with N-(4-methylbenzenesulfonyl) -N'-n-butylurea, a wellknown blood sugar level depressant which is widely used in medicine.

The several sulfonamide compounds listed in Table II are again identified by the substituents A and B in Formula 1.

It is seen that the sulfonamide compounds of the invention compare favorably with the known urea derivative in their effectiveness and their acute toxicity. The important ratio between the lethal dosis and the percentage reduction obtained by a fixed dosis is more desirable for are obvious to those familiar with the art of pharmacy.

While the invention has been described with particular reference to specific embodiments, it is to be understood that it is not limited thereto, but is to be construed broadly and stricted solely by the scope of the appended claims.

most of the novel compounds than for the known product. 5 We claim:

In a preparation intended for prolonged medication, 1. 2 tetrahydrofurfurylsulfonamido-S-methoxypyrimithe consequences of prolonged ingestion are important. dine. Table III lists the results of autopsies performed on rats 2. 2 tetrahyd-rofurfurylsulfonatmido S-ethoxypyrimiwhich had been given blood sugar depressants of this in- 10 dine. vention per os for four weeks in daily doses of 100 3. 2 tetrahydrofurfurylsulfonamido-S-isobutoxypyrimrug/kg. Ten rats were used in each test, and one group idine. of ten rats was given N-(methylbenzenesulfonyl)-N-n- 4. 2 methallylsulfonamide-S-ethoxypyrimidine. butyl urea for the purpose of comparison. 5. Z-n-butylsulfonamido-S-methoxypyrimidine.

The subacute toxicity of the compounds tested was 15 6. Z-n-butylsulfonamido-S-ethoxypyrimidine. evaluated from histological examination of the livers of 7. 2-n-butylsulfonamido-5-isobutoxypyrimidine. the test animals. 8. 2-isoamylsulfonamido-S methoxypyrimidine.

TABLE III Number of Nature of pathological Compound rats with liver changes damage N- (methylbenzenesulionyl) -N-n-butyl urea.

2-isoamylsulfonamido-S-methoxypyrimidine 2-benzenesullonamido-an-propoxypyrimidine so Hea Major necroses and necrobioses.

Minor necroses.

Minor degenerative liver changes.

No changes.

No changes.

The sulfonamides of the invention tested for subacute toxicity are better tolerated than the known compound which is in daily clinical use. The differences in toxicity between the tested compounds of our invention and the known compound are very substantial, particularly when it is considered that all the compounds of the invention listed in Table III have stronger blood sugar lowering efiects than the known compound.

The salts of the sulfonamide compounds of our invention have the same physiological effects as equimolecular amounts of the free compounds. There is no detectable difference in activity between the free compounds and the several physiologically tolerated metal salts and salts of organic bases and of ammonium which are mentioned hereinabove. The effects of the sulfonatmide compounds of the invention are not influenced by the usual carriers and adjuvants employed in pharmaceutical praxis. They are equally effective when orally ingested as powders, tablets, in d-ragees, capsules, pills, in suspensions or solutions, except for those delaying efiects of the carrier which '9. 2-isoamylsulfonamido-S-ethoxypyrimidine. 10. 2-isoamylsulfonamido-S-n-propoxypyrimidine. 11. 2-ethoxyethylsulfon-amido-5-isobutoxypyrimidine.

pyrimidine.

16. 2 n butoxyethoxyethylsulfonamido S-methoxypyrimidine.

17. 2 ethoxyethoxyethoxyethylsulfonamido-S-ethoxypyrimidine.

No references cited.

ALEX MAZEL, Primary Examiner.

MARY U. OBRIEN, Assistant Examiner. 

1. 2 - TETRAHYDROFURFURYLSULFONAMIDO-5-METHOXYPYRILMIDINE. DINE.
 5. 2-N-BUTYLSULFONAMIDO-5-METHOXYPYRIMIDINE.
 15. 2 - ISOPROPOXYETHOXYETHYLSULFONAMIDO - 5-METHOXYPYRIMIDINE. 